Unique Aspects of Laboratory Testing
- Comprehensive antibody screening using purified HLA molecules is available on multiple platforms (ELISA, flow cytometry, Luminex)
- Microarrays with single HLA molecules provide the most sensitive and specific assays available
- Epitope prediction by correlation with protein sequences is available
Tests
SOLID PHASE METHODS
- HLA Class I PRA
- HLA Class II PRA
- Antibody Specificity Single Antigen ID, Class I
- Antibody Specificity Single Antigen ID, Class II
- HLA Class I Antibody Analysis with Titer
- HLA Class II Antibody Analysis with Titer
- ELISA Antibody Screen
CYTOTOXICITY
- Quick Screen PRA T lymphocytes
Indications
SOLID PHASE METHODS
- To detect HLA antibodies in recipients for selection of HLA mismatched donors
- To identify compatible HLA types for transfusions
- To detect HLA antibodies after transplantation
- To detect IgG antibodies
CYTOTOXICITY
- To detect antibodies against T lymphocytes
- To detect IgM and IgG cytotoxic antibodies
Methods
SOLID PHASE METHODS
- For bead array methods, purified HLA molecules are attached to microparticles. Binding of antibodies is detected by a flow cytometer or Luminex instrument.
- For ELISA methods, purified HLA molecules are attached to wells. Binding of antibodies is detected by ELISA methods.
- Purified HLA molecules consist of one of the following:
(1) Mixtures of HLA molecules from many donors
(2) HLA molecules from single donors
(3) Single HLA molecules (single antigens)
CYTOTOXICITY
- Microcytotoxicity with anti human globulin to enhance sensitivity
Turn Around Time (arrival before 10am)
SOLID PHASE METHODS
Routine test results in 5-15 business days
STAT testing in 3 business days
CYTOTOXICITY
Routine test results in 3-5 business days
STAT testing in 2 business days