Unique Aspects of Laboratory Testing
- High resolution types are resolved to a SINGLE allele.
- Homozygous types are confirmed using at least two typing methods, family pedigree or linkage analysis.
- Rare alleles and unusual combinations of alleles are confirmed.
- Novel alleles are fully characterized using selective amplification or cloning.
- High resolution typing is performed using automated nucleotide sequencing supplemented with SSP and SSO methods as required to resolve ALL ambiguities.
- Expert consultation is available for all donor searches and selection.
Single Allele Resolution
Tests
High resolution typing is available for the following loci:
HLA-A HLA-DRB1 HLA-DPB1
HLA-B HLA-DRB3/4/5 HLA-DQA1
HLA-C HLA-DQB1 HLA-DPA1
Reduced cost for packages:
A, B, C, DRB1 and DQB1
A, B, C, DRB1, DQB1 and DPB1
A, B, C and DRB1
Indications
- To determine HLA matching between transplant recipient and
donor(s) - To confirm HLA identity for related individuals
- To identify HLA types required for immunotherapy
- To diagnose certain HLA-associated diseases
Methods
- Sequencing
- SSO, SSP and/or Cloning to resolve ambiguities
Turn Around Time (arrival before 10am)
Routine test results in 4-10 business days
STAT testing in 2-3 business days
Low/Intermediate Resolution
Tests
Low/Intermediate resolution typing is available for the following loci:
HLA-A HLA-DRB1
HLA-B HLA-DQB1
HLA-C HLA-B27
Reduced cost for packages:
A, B and C
DRB1 and DQB1
Indications
- To determine HLA types of family members
- To screen unrelated donors for bone marrow transplantation
- To evaluate donors for solid organ transplants
- To diagnose certain HLA-associated diseases
Methods
- SSO and SSP
Turn Around Time (arrival before 10am)
Routine test results in 2-5 business days
STAT testing in 1-2 business days
Serology
Tests
HLA-A, -B and -C
HLA-AB Expression (confirms expression of DNA-based HLA types determined by DNA typing performed by this lab)
Indications
Confirming expression of types determined by DNA-based method
Methods
-
Microcytotoxicity
Turn Around Time (arrival before 10am)
Routine test results in 2-4 business days
STAT testing in 1 business day